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Syllabus
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Instructor: Leslie Lichtenstein
Office:
S542 Ext. 1618
Email:
llichtenstein@massasoit.mass.edu
Web site: http://www.massasoit.mass.edu/faculty/llichten
Online course: http://www.massasoit.mass.edu:8080/
Prerequisite: Anatomy and Physiology I &
II, or a grade of "C" or better in
Biological Principles.
Credits:
4
TEXTS:
Microbiology, 6th Edition, by Totora, Funke and Case
Microbiology Atlas and Exercises, Leboffe and Pierce; Morton Publishers
Grading policy: Exams=45%
Labs=35% Final exam=20%
(N.B.You MUST pass the laboratory to pass the course.)
COURSE OBJECTIVES:
In this course we will look at bacteria, viruses, fungi, protozoa and
some metazoans. Emphasis will be on practical applications for medical,
food, water and industrial applications of these organisms. Students will
be expected to develop a familiarity with all organisms and acquire the
proper laboratory techniques to enable them to identify unknown organisms.
ATTENDANCE:
Students are very strongly urged to attend all lectures and laboratory
sessions. Missed labs must be made up. Consult the college catalog for
MCC attendance policy - only 5 hours of absences are allowed.
ASSIGNMENTS:
Students are expected to understand all lecture and laboratory materials.
If you don't, ASK!!!
Every student is required to keep a lab notebook. Lab exercises should
be read before lab. When we begin unknowns, you should have WHY you are
doing each test writen in your notebook before the next lab session meets.
Try to keep up with the reading schedule.
TEACHING FORMAT:
This class is taught with a lecture format using the blackboard, overhead
projector and other audiovisual aids, some demonstrations and class discussion
when appropriate. Resources & assignments will be found on the course
website. We will become familiar with and utilize material on the WWW in
this course. An online summary version of the text is available on the
MCC server where quizes may be taken.
LECTURE SCHEDULE
WEEK TOPIC
CHAPTER
1
History & Evolution
Preface
Diversity of microorganisms
1
Classification
10(pp 268-272)
(Review chemistry from Bio Principles at home- we do not
cover this in class.)
2
Protists - Algae
12(pp339-343)
Protozoa (see specific organisms)21-26
3
Helminthes & Arthropod Vectors
12(344-353)
(see specific organisms in 21, 23, & 25)
Fungi
12(320-329)
(see specific organisms in 21,22,24,25 & 26)
4
Nutritional Requirements
6
EXAM (1,10 & 12)
5
Morphology
4
Metabolism
5
6
Viruses
13
(see viral diseases in chapts. 21-25)
7
Genetics
8
Recombinant DNA
9
8
Intro to Medical Bacteriology
10(276-278)
Classification & Bergey's
11
Gp II Gm + (see specific organisms in 21-26)
EXAM (4,5,6,8,9 & 13)
9
Gp I Bacteria Gm-
Gp III & IV
10
Principles of disease
15
11
Epidemiology
14
Physical & chemical controls
7
12
Antimicrobials
20
13
EXAM (10,11,14,15, & 20)
Environmental Micro
27
14
Industrial & Food Micro
28
LABORATORY OUTLINE
1
Lab Saftey Intro
Microscopy (Read Totora pp 56-67)
Ex 3.1 & 3.2
Media
(Read Totora pp 162-167) Ex. 1.1
2
Protozoa, Algae & Cyanobacteria pp24-30 Ex 10.2
Parasitic Protozoa
Exposure plates
3
Helminthes
Ex 10.3
Fungi
Ex 10.1
4
Read microbial transfers (1.1 & 1.2)
Streak plates
Ex. 4.1
Lab Practical
5
DNA Extraction
BioTech-Electrophoresis
Transformation
6
Smear prep (Read Totora pp68-71)
Ex 3.3
Simple staining
Ex 3.4 & 3.5
Gram staining
Ex. 3.6
7
Spore stain
Ex 3.9
Acid-Fast stain
Ex. 3.7
8
Identification of unknowns read 5.5
Culture techniques (Read Totora pp278-281)
Culture characteristics
Ex. 2.1-2.4
Read - Use of Bergeys Manual
Ex 6.1 & 6.2
9
Gm + Unknown Ex. 1.3, 5.16, 5.18,
5.20
10
Biooxidations & Hydrolysis Ex. 5.1,5.2,5.4,5.6,5.9,5.11,5.15
11
Gm- Unknowns Ex. 4.6,5.7,5.10,5.12,5.23,5.22,5.27
API-20E
Motility, MRVP
Ex. 3.11
12
Antibiotics
Ex. 8.3
13
Physical & chemical controls
Ex 8.1
14
Food Micro
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Revised: March 27, 2001.