Massasoit Community College
Biology Department
Microbiology Syllabus – Biol 231-01
Fall 2003
INSTRUCTOR: Debra St. George
OFFICE: s-323
TELEPHONE: 508-588-9100, Ext. 1617
E-MAIL: dstgeorge@massasoit.mass.edu
OFFICE
HOURS: Monday and Wednesday 9:00-9:45
a.m.
or by appointment
texts:
Microbiology, An
Introduction, 8th edition; Tortora, E., Funke, B. and Case, C.
2001. San Francisco; Benjamin Cummings
Publishing Co., Inc.
Lab Manual: Microbiology,
A Photographic Atlas for the Laboratory, Alexander, S. and Strete, D. 2001. San Francisco: Benjamin Cummings Publishing Co., Inc.
Study Guide: To accompany text, (optional) chemistry
Review Book (optional)
MATERIALS:
1. Notebook to take notes and keep assignments and/or handouts.
2. Composition book, pages attached (sewn in and numbered), at least
100 leaves.
3. Red and blue pen and pencil.
Please bring to lab and store in your draw.
COURSE
DESCRIPTION:
This is a course in general
microbiology with emphasis placed on the practical applications for medical, food, dairy, water, and environmental
microbiology. Part of the laboratory
experience includes an introduction to techniques in molecular biology and the
identification of one or more bacterial “unknowns” to demonstrate adequate
knowledge of the proper laboratory technique.
Organisms of discussion include bacterial, viruses, fungi, and some of the primitive
algae, metabolism, microbial
growth, control of microbial growth, and microbial genetics are also
introduced.
4 credits
Prerequisite: Grade of “C-” or better in
Biological Principles (31121) or successful performance on departmental
challenge exam, and Preparing for College Reading II (11092), Introductory
Writing (11099), and Fundamentals of mathematics (38010), or waiver by
placement testing results, or Departmental approval. Chemistry is recommended but not required.
TEACHING PROCEDURE:
Teaching will be done as a
combination of formal lecture with aid of overhead transparencies, discussion
and demonstration of techniques in the laboratory portion of the course.
MICROBIOLOGY Syllabus – 31231 Page
2
Prof. St.
George
COURSE
OBJECTIVES:
1. Provide solid introduction to microbiological principles upon
which the student may build.
2. Develop skills necessary to function in a microbiology
laboratory.
3. Demonstrate problem solving ability and the capability to locate
helpful resources when needed.
4. Address topic objectives listed in the text.
EVALUATION:
Comprehensive final
exam.................................................... 20%
Three (3) Section Exams....................................................... 40%
Laboratory Unknowns........................................................... 20%
Aseptic Technique, Attendance, Punctuality,
Equipment
Usage Evaluation................................................. 10%
Lab Reports and Log............................................................. 10%
GRADING:
A = 100-93 B+
= 89-87 C+ = 79-77 D+ = 69-67
A- = 92-90 B = 86-83 C = 76-73 D = 66-63
B- = 82-80 C- = 72-70 D- = 62-60
F = 59 or below
COURSE POLICIES:
1. Make-up exams will be given at the discretion of the instructor
and at a time that is mutually convenient for the instructor and the
student. Due to the sequential nature
of this class, make-ups should be completed within one week.
2. Students are expected to attend all class meetings on time. There will be three (3) hours of unexcused
absences allowed in this course. Any
absences above the three (3) hours could result in a lowering of the student’s final
grade. Attendance will be taken at the
start of each class session.
Punctuality and absences will be recorded. It is the student’s responsibility to make up any missed work.
3. Lab work is an integral part of the course. Students must do the lab work in class to get credit for it. Lab work passed in late could result in a lower grade.
4. Please do not disrupt class with cell phones or beepers. If necessary, please keep them on vibrate or
display and take calls outside class.
Microbiology (Biol
231-01) Course Outline
|
Week
Of |
Week
# |
Topic |
Test |
Book Chapter |
|
9/03 |
1 |
Introduction and History Survey, Microscopy |
|
1, 3 TFC |
|
9/08 |
2 |
Chemical Principles, Prokaryotes |
|
2, 4 TFC |
|
9/15 |
3 |
Eukaryotes Fungi/Algae Mycobacteria |
|
4,13 TFC |
|
9/22 |
4 |
Parasitology |
|
12 pgs 356-363 &
349-356 23 pgs 647-649 25 pgs 706-707 &
708-712 |
|
9/29 |
5 |
Gram Stain Media Culturing Bacteria |
Test |
3, 6 TFC |
|
10/6 |
6 |
Gram Positive Biochemicals Gram Negative Biochemicals |
|
5 AS |
|
10/13 |
7 |
Classification Viruses |
|
10, 13 TFC |
|
10/20 |
8 |
Metabolism |
|
5 TFC |
|
10/27 |
9 |
Bacteria & Arcgaea |
|
11 TFC |
|
11/3 |
10 |
Microbial Genetics |
Test |
8 TFC |
|
11/10 |
11 |
Microbial Genetics Recombinant DNA |
|
8, 9 TFC |
|
11/17 |
12 |
Recombinant DNA Antibiotics |
|
9, 20 TFC |
|
11/24 |
13 |
Antibiotics API |
|
20 TFC Handout |
|
12/1 |
14 |
Chemical Control of
Microorganisms |
Test |
7 TFC |
|
12/8 |
15 |
Principles of Disease |
|
24 TFC |
|
12/15 |
16 |
Microbial Pathogens |
|
9 AS |
TCF = Totora,
Funke, Case
AS = Alexander,
Strete
Week of
|
Week #
|
Topic |
|
9/03 |
1 |
Safety Record Keeping |
|
9/08 |
2 |
Hand Washing Experiment Wet Preps |
|
9/15 |
3 |
Wet Preps Fixed Smears Acid Fast Stain |
|
9/22 |
4 |
Protozoa Helminths |
|
9/29 |
5 |
Broth Gram Stain Sub Culture Colonial Morphology |
|
10/6 |
6 |
Broth Gram Stain Sub Culture Set-Up & Read Gram Positive Biochemicals |
|
10/13 |
7 |
Set-Up & Read Gram Negative Biochemicals |
|
10/20 |
8 |
Unknowns |
|
10/27 |
9 |
Unknowns |
|
11/3 |
10 |
Unknowns |
|
11/10 |
11 |
Complete Unknowns Bacterial Transformation |
|
11/17 |
12 |
Spore Stain Kirby Bauer Sensitivity |
|
11/24 |
13 |
Set-Up & Read API |
|
12/1 |
14 |
Set-Up & Read Exposure Plates |
|
12/8 |
15 |
Media Preparation |
|
12/15 |
16 |
Make-Up day |